Step 1: Connect the camera to microscope and switch the microscope ON.
Step 2: Now install software from CD or Pendrive whichever is provided my the company.
Step 3: You need a scale to calibrate microscope. For stereo-zoom microscope use scale. For other type of microscope use an ocualar micrometer.
Step 4: Now place the ocular micrometer on the stage. Change the magnification from smaller to larger and take a snap for every magnification and save it with .tft. Saving the images in .tft format makes our work simpler. We can directly do the calibration from the images.
Brief summary is that you switched ON microscope and connected camera to it and installed software on your system. Place ocular micrometer and take snaps at different magnifications and save it with an extension of .tft.
Step 5: Open the snaps with .tft files. Go to layers—> calibrate.
Step 6: A dialogue pops up along with a red line on the image.
Brief summary is that you saved the image with .tft format and opened it in the software.Make sure your resolution is 100%. Click on calibrate in layers menu. Now you have a red line on the image and a dialogue opened.
Step 7: Place one end of the line on the starting point of a reading line and other end of line on the starting point of the other reading line. Make sure you know the reading between two lines on the scale.
Step 8: Now select the magnification on the pop up dialogue. Write the actual length of the reading in actual length. Notice the units and type the value in it. Repeat the process till you do for all the other magnifications.
You can verify the process by placing the red line on the known distance. If it shows any error you can redo the process from .tft file. You can also use .tft files on other system to calibrate as well.